http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102532312-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_89d8e7355750aa815842a3fa5b382c19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-02 |
| filingDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d506a0daf7438da560a111c9a266aa8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc553ba04d9c2c88292b3f61d9d53443 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3209f46c30746534b254bbe912da0f45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d38067a527e2149254edc311a1315a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_197566246e7b0e828e640af246b3989e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7acd2154d75e189855de1dfcd32fa8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86f86572282b0205b4b479492c6abe34 |
| publicationDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102532312-B |
| titleOfInvention | Anti-Vibrio anguillarum yolk antibody, and preparation method, titer detection method and application thereof |
| abstract | The invention discloses an anti-Vibrio anguillarum yolk antibody, and the preparation method, the titer detection method and the application thereof. The invention is characterized in that the anti-Vibrio anguillarum yolk antibody is obtained by immunizing laying hens with Vibrio anguillarum as antigen, collecting highly-immunized eggs and performing separation and purification of yolk of the immunized eggs. The method comprises a step of preparing a Vibrio anguillarum antigen and inactivating the antigen, a step of performing basic immunization once in laying hens and secondary immunization for two to three times and collecting eggs, and a step of performing centrifugal separation, salting-out and dialysis to obtain the final product. The titer of the anti-Vibrio anguillarum yolk antibody is determined by indirect enzyme-linked immunosorbent assay. The anti-Vibrio anguillarum yolk antibody can be used for prevention and treatment of diseases caused by Vibrio anguillarum infection and can also be used for development of Vibrio anguillarum test kits and immunoreaction and diagnosis in immunology. The invention has the advantages that the yolk antibody is higher in purity and has a significant inhibitory effect on Vibrio anguillarum; the extraction of highly-immunized yolk is cost-efficient and convenient, and is adapted to low-cost and mass production; and the preparation method is simple and feasible. |
| priorityDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.