http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102517369-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b3b518e0b6562f18cd81e63ccafc7d4d |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-40 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P39-00 |
filingDate | 2011-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4c17296d94ad7ce586728828b823455 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a647ed6ee9bd011b0b9141f2f0a2fc6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_116663643c7fee54ca22488534227e5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2aef5ffc1e79b313cafc35f2888dd95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7aa8d34e78467901567bc90d10efbdb7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3057fce0819b29ea612f07df14df6353 |
publicationDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102517369-B |
titleOfInvention | Method for synthesizing benzene-containing polyhydroxyalkanoate by mixed fermentation of high-density microbes under nitrogen restriction |
abstract | The invention discloses a method for synthesizing benzene-containing polyhydroxyalkanoate by mixed fermentation of high-density microbes under nitrogen restriction. The method comprises the following stages of: 1, adding less than or equal to 0.5g/L of benzene-containing organic substance and 5 to 20g/L of sugar serving as a carbon source into a fermentation medium, inoculating a seed solution oftwo or more microbes, judging the consumption of the carbon source by using online signals, and adding the carbon source to ensure the growth requirement of the microbes; and 2, regulating the pH value by using a nitrogen-containing alkali solution when the light absorption density of microbial cells is 30, and stopping supplying aqueous ammonia and other nitrogen-containing substances so that the nitrogen content of the fermentation solution is reduced to 0.001mol/L and the nitrogen restriction is realized; and monitoring the change of dissolved oxygen and the pH value, and thus obtaining the benzene-containing polyhydroxyalkanoate when the dissolved oxygen and the pH value are simultaneously raised and the carbon source is converted. By the method, the benzene-containing polyhydroxyalkanoate containing short-chain monomers and medium long-chain monomers is synthesized by microbial mixed fermentation and sectional supply of multiple carbon sources, and the composition of the product can be controlled in a large range. |
priorityDate | 2011-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.