http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102517281-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_161663de5408e572e0d7c6b04dfabc7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1f8e19cdfdca567075a7aa21955b87d4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2011-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e45a216984136ceb2f5d78a99a1c1c3a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95e1dc8b53a77a0614db57313883e532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78d7ff4f1c545c2df895482c3676d568 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7fa9e9b29f34c5c30b5f3ee85f182f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_107fff25d5e80aba9f0b0a263c34972c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f307ed55e92210b44ba3b2371b36d54 |
| publicationDate | 2013-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102517281-B |
| titleOfInvention | Tetrahymena expression vector of chitinases and its application in expressing chitinases |
| abstract | The invention discloses a Tetrahymena expression vector of chitinases and its application in expressing chitinases. The invention provides an expression cassette, which includes an HSP70-2gene promoter as shown in the 9th-1126th nucleotides from the 5' terminal of sequence 1, an encoding gene of chitinases in sequence 2 and an HSP70-1 gene termination signal as shown in the 3835th-4312th nucleotides from the 5' terminal of sequence 1. The invention also provides a recombinant plasmid containing the expression cassette. When transferred to a Tetrahymena through electroporation, the recombinantplasmid can, under the drug screening effect of paromomycin, replace most of the original rDNA in the Tetrahymena, and multiply an exogenous gene in the Tetrahymena massively, thus realizing genetic transformation of a target gene. Under heat shock conditions, the HSP70-2 strong promoter can start the high level expression of CBD3 gene, and the expressed chitinases have bioactivity (able to hydrolyze chitin). In conclusion, the expression vector of the invention has wide application prospects. |
| priorityDate | 2011-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.