http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102517276-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 |
filingDate | 2011-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69cadb708af24fd054bec3591b3efaae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_356ec39ba440c82ee0fea2311af6e51b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_452af5f2ca9ed5228198ab18eedb7d56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_829a2f17add5bb12b33ea02052219b76 |
publicationDate | 2013-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102517276-B |
titleOfInvention | Method for preparing magnetic nano carrier immobilized aldolase with high substrate tolerance |
abstract | The invention discloses a method for preparing magnetic nano carrier immobilized aldolase with high substrate tolerance. The method comprises the following steps of: 1) preparing super-paramagnetic Fe3O4 nano particles by co-precipitation of a mixture of ferrous and ferric iron salts, modifying the particles by using a silane coupling agent, and activating the outer surfaces of the modified particles by using glutaraldehyde to obtain surface activated magnetic nano particles; 2) stirring a purified and desalted aldolase buffer solution and a magnetic carrier at a low temperature, washing, performing freeze drying, and thus obtaining the magnetic nano carrier immobilized aldolase; and 3) catalyzing 2-deoxy-D-ribose-5-phosphoric acid by using the magnetic nano carrier immobilized aldolase as a catalyst to obtain 3-glyceraldehyde phosphate and acetaldehyde. In the reaction of catalyzing the 2-deoxy-D-ribose-5-phosphoric acid by using the immobilized aldolase, the tolerance of the acetaldehyde substrate is remarkably improved, and the recycling operation of enzyme is greatly simplified. |
priorityDate | 2011-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 206.