http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102507470-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_30783f5e0439f26fb23d0089d47f0ea6 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 |
| filingDate | 2011-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbe6725a31eed8ed7b5c8c0728afa96e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f064169ef24d535f3a202016b6e8079d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77a8464f8be3838bb2a88bc31a3b4d58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa3237bc184a0f2fcdc3f6b901d303eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7d01a84fead5db577262235614d64a8 |
| publicationDate | 2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102507470-B |
| titleOfInvention | Method for measuring enzyme substrate amount by combining enzyme reaction process method and terminal balance method |
| abstract | The invention relates to a method for measuring enzyme substrate amount by combining a terminal balance method and an enzyme reaction dynamic process to process enzyme reaction process data. The method comprises the steps: the data, which has the characteristic that the signal change between adjacent data exceeds two times of the noise of an instrument, in a continuously recorded enzyme reaction curve is valid data; detection signals are measured before little enzyme liquid is added, and an enzyme liquid dilution effect is corrected to obtain a corrected starting signal; when the number of the valid data in the enzyme reaction curve is not more than 7 or the absolute value of the difference between the corrected starting signal and the final recorded data is smaller than 50 times of the noise of the instrument, a reaction terminal signal is determined by the terminal balance method, and when the number of the valid data is more than 7 or the absolute value of the difference between the corrected starting signal and the final recorded data is larger than 50 times of the noise of the instrument and the consumption ratio of the substrate achieves requirement, the reaction terminal signal is predicted by an enzyme reaction process analysis method; and the concentration of the substrate to be measured is determined according to a standard response curve with the absolute value of the difference between the corrected starting signal and the reaction terminal signal as a measuring index. The method is applicable to irreversible enzyme reaction systems which can be continuously monitored. |
| priorityDate | 2011-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 235.