http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102492777-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5ed85c5a886b8df4923d9a2596262902 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75ff163ef0aa449de58fa9291b3b8518 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3654f342eca76b6f892414fba9994cda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a11e84759a8cc6cda784efedbb9a114 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3008a9f0c988fa48090655254b8c8fb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2ae9839b1fda2c5dabae12219c60c27 |
| publicationDate | 2013-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102492777-B |
| titleOfInvention | Standard plasmid molecule for transgenic maize Mon810 detection and construction method thereof |
| abstract | The invention belongs to the technical field of biological detection, and particularly relates to a standard plasmid molecule for genetically modified maize strain Mon810 detection and a construction method thereof. The standard plasmid molecule comprises a strain specific sequence, a construction specific sequence and a maize internal standard gene zSSIIb specific segment of genetically modified maize strain Mon810; by analyzing an exogenous insertion vector border sequence of the genetically modified maize strain Mon810 and a sequence of a joining region of two elements Hsp70 and cryIA (b), strain specific and construction specific PCR (Polymerase Chain Reaction) primers are designed, amplification is performed to obtain the strain specific segment and the construction specific segment of the genetically modified maize strain Mon810 and the maize internal standard gene specific sequence; and an artificial recombinant plasmid molecule pMHZ is obtained by constructing the sequences and the segment into the plasmid molecule through a molecular cloning method. A positive standard sample of the genetically modified maize strain Mon810 can be fully substituted by the constructed standard plasmid molecule; and the plasmid molecule is used for quantitative PCR analysis and detection of the genetically modified maize strain Mon810 sample. |
| priorityDate | 2011-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.