http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102492771-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d8438018a20cfe60179ea22f19b11742 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49c9bf04efb87cb8ea8eb105c2f735a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d655c5db44e078024d87362777ff2293 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51cbc495a98e827080c09225ea2ef965 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dad70e329ebf4aa439c0c59b0a3de4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8cf1cf2af125f755522fe2f80ec51078 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bd5ef7361d573d950dd5a2060782afa |
| publicationDate | 2013-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102492771-B |
| titleOfInvention | Method for constructing chromosome physical map of rose plant |
| abstract | The invention relates to a method for constructing a chromosome physical map of a rose plant, which comprises the following steps: preparing a mitosis metaphase chromosome slide through an improved enzymolysis and deplasmolysis low-permeability method, wherein in the probe marking process, a nick translation method is adopted, and digoxin-modified nucleotide is used to mark plasmid DNA (deoxyribonucleic acid) carrying target genes and enable the DNA to become a hybridization probe; and then, directly hybridizing a chromosome-denatured DNA single chain with the DNA probe, and carrying out specific immune reaction on anti-digoxin antibody coupled with rhodamine and the digoxin marked on the probe to detect the positions and distribution of the target genes on the chromosome, thus obtaining the chromosome physical map. The invention can carry out fluorescent in-situ hybridization on a rose plant which has a small chromosome and many cell inclusions, and can stably obtain a clear chromosome physical map. Besides, the method is universally applicable and easy to operate with respect to a rose plant. |
| priorityDate | 2011-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 131.