http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102485752-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a8ccdc728182235c0ba3fd2e8d20ea6 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-59 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate | 2010-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62d098855c4f294947ee99b63e1f202f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b807c70cdb37466cd76ca15a0aa0beb6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c4ed07a906a71761f74efd3c245d8eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05af071db31ade5367d6e2e04a8d2c97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1e560c1d973e4ed5f53c1686405d4e0 |
| publicationDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102485752-B |
| titleOfInvention | Purification method for human chorionic gonadotrophin (HCG) |
| abstract | The invention provides a purification method for HCG. The purification method comprises the following steps: 1, dissolving crude HCG in a 0.05mol/L sodium acetate buffer with the pH value of 4-5, adding the obtained solution to a carboxymethyl cation exchange chromatography column with agarose, sephadex or basic calcium phosphate as a carrier, eluting with a buffer which has a pH value of 4-5 and comprises 0.05mol/L sodium acetate and 0.2-0.5mol/L sodium chloride, and collecting the eluate with the optical density of 0.6-0.7 at 270-290nm, and preferably 280nm; 2, desalination-concentrating the eluate; and 3, adding to the concentrated eluate to the carboxymethyl cation exchange chromatography column, eluting with a 0.05-0.1mol/L sodium acetate buffer with the pH value of 5.5-6.5, and collecting the elute with the optical density of 0.6-0.7 at 270-290nm, and preferably 280nm. According to the purification method, the HCG titer of HCG products is not less than 7000iu/mg, and the total yield exceeds 90%. |
| priorityDate | 2010-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.