Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
filingDate |
2010-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee897db5a75f982775a6fe76dad345b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f65a980d9419d727b50e33679ca4defb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb7192425960862b6917853373407e71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af3a776af6d1f05c147e8ad715707c7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3e35897234cba21b22b6369f81704c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0277eef18c4b90bca20718ab9b58e5be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee033ef434e23f50889141a70bf46d46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0aef66a65563066a01479c59a4fc679 |
publicationDate |
2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-102464716-B |
titleOfInvention |
ELISA (Enzyme-Linked Immuno Sorbent Assay) kit for detecting Japanese encephalitis virus antigens in swine, human and mosquitoes and application |
abstract |
The invention belongs to the technical field of animal virology and immunology, in particular to a double-antibody sandwich ELISA (Enzyme-Linked Immuno Sorbent Assay) kit for detecting Japanese encephalitis virus antigens in swine, human and mosquitoes. Core reagents of the kit comprise a Japanese encephalitis virus E protein monoclonal antibody serving as a primary antibody and a rabbit polyclonal antibody serving as a secondary antibody. The invention discloses a preparation and purification method of the Japanese encephalitis virus E protein monoclonal antibody and the rabbit polyclonal antibody. The invention also discloses a detection method of the double-antibody sandwich ELISA. A hybridoma cell strain secreting the monoclonal antibody is preserved in China Center for Type Culture Collection with CCTCC NO. C2010114. |
priorityDate |
2010-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |