http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102455360-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d5a61413fafd43fa43c91bfc7b85d1da |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2010-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f25ff0367a04be317ef2511b3478d6d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09863cce2bff0c82776080618f251faa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6543fd4aea850eea3d50803db8c0d71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ca554a1bc0afe3edf888042aeb4e952 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edc90f4312c6e761420db40ade81ab58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a2689217693ed97fac96b208fae6769 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_efed9c6f4c94f4c74e666d3c9634c042 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1720cde0339edeb7eba77bc67ce3397 |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102455360-B |
| titleOfInvention | Neosporosis recombinant enzyme-linked immunosorbent assay (rELISA) antibody assay kit |
| abstract | The invention provides a neosporosis rELISA antibody assay kit. The neosporosis recombinant enzyme-linked immunosorbent assay (rELISA) antibody assay kit is characterized in that designing a primer set according to a neospora caninum NcSRS2 gene of the GenBank, introducing the primer set to an enzyme digestion site, removing a signal peptide zone of an NcSRS2 protein, extracting a DNA from Xinjiang cow positive serum, carrying out polymerase chain reaction (PCR) amplification of the DNA to obtain a tNcSRS2 genetic fragment having length of 975bp, cloning the tNcSRS2 genetic fragment into a pMD-18-T carrier, carrying out enzyme digestion and sequencing, further directionally connecting the desired fragment obtained by the previous step to a pGEX-4T-2 expression carrier, transferring the pGEX-4T-2 expression carrier with the desired fragment into escherichia coli, carrying out inducible expression and purification, wherein expressed fusion protein molecular weight is about 62kDa, and carrying out ELISA antigen coating by the expressed fusion protein to obtain the neosporosis rELISA antibody assay kit. A use method of the neosporosis rELISA antibody assay kit comprises the following steps that a coated enzyme label reaction plate is taken out, is subjected to first washing, is enclosed, is subjected to second washing, is added with a sample needing to be detected, is subjected to third washing, is added with a secondary antibody, is subjected to fourth washing, and undergoes a color reaction; and when the color reaction is finished, an OD450/630 value of the coated enzyme label reaction plate is read and a result is determined. Compared with a commercial ELISA assay kit, the neosporosis rELISA antibody assay kit has a higher coincidence rate above 95% The neosporosis rELISA antibody assay kit also is suitable for the ELISA of a neospora caninum antibody in ruminant serum and blood plasma. |
| priorityDate | 2010-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.