http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102453093-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2010-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dec5460656c3e4d58ddb9632a14963f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03d37159410dfdebf167a3a37c997dfc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ef8efe201681a514c4b37793a8a1420 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_406f4e39e1c86130fb0e6a23f7019be6 |
| publicationDate | 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102453093-B |
| titleOfInvention | Screening and application of single-chain antibody against fumonisin |
| abstract | The invention belongs to the technical field of genetic engineering, and in particular relates to screening of a single-chain antibody against fumonisin and application thereof in immunoassay of fumonisin. The screening method comprises the following steps of: directly setting off from coupled antigen FB1-KLH(keyhole limpet hemocyanin) immunized mouse spleen cells, establishing a single-chain antibody gene library by a molecular cloning method and technology, then screening by a phage display technology, expressing ELISA(enzyme-linked immuno sorbent assay) test, sequencing, and finally obtaining the single-chain antibody against fumonisin named as FB-Mu 1H3 with high affinity and a coding gene thereof. The single-chain antibody can be directly applied to assay of fumonisin after being expressed and purified in a large quantity in escherichia coli, and the applications include assay of fumonisin pollution in field crops, feed, grain or food. |
| priorityDate | 2010-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.