| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259045c6eba6e2c5d338b84e0a88b571 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-587
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-588
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-52 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
2010-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b3633841f3d2a4fb0f111a0cea22052
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a29ef82fa4dd9ab462be4d2b1d24284
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eff88edf3be552134315f1778f528e58 |
| publicationDate |
2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-102449485-B |
| titleOfInvention |
Method for detecting a ligand using fret biosensor |
| abstract |
The present invention relates to a method for detecting a ligand using a fluorescence resonance energy transfer (FRET) biosensor. More particularly, the present invention relates to a method for simply detecting a ligand within a specimen by measuring the FRET of the biosensor while maintaining a critical temperature, using a phenomenon in which the ligand-binding protein constituting the biosensor is reversibly unfolded at a temperature higher than a specific critical temperature, and the level of such reversible unfolding varies in accordance with the concentration of the ligand. The method of the present invention can be widely applied to various types of FRET biosensors using ligand-binding proteins. |
| priorityDate |
2009-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |