http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102443543-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_550246a1a90f3c3034cf90d3392188d9 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 |
| filingDate | 2011-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c98273039372155d122615d64119f6ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef0334f10ab143b772ef0fe01c90c385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad4428e31b8cec0d5fa8bd9502432600 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_baee1bfb4cc852afb43e1a51f546e626 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fb98a3526f5ca000a62ad66db799c27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de3aadd6cca8adffcb51b66536a8b502 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7c999c28c9c21f1159ab7595a2884f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cc195dfe102c2bec8a173711fa94b73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11d6d704b23f4351d940efd22f9f4eb4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fcd18241e1e5acb954af2fea93436ad |
| publicationDate | 2014-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102443543-B |
| titleOfInvention | Anti-white-spot-syndrome-virus transgenic algae strain as well as preparation method and application thereof |
| abstract | The invention relates to an anti-white-spot-syndrome-virus transgenic algae strain as well as a preparation method and an application thereof. The natural relationship among shrimps, white spot syndrome virus (WSSV) and dunaliella cells is ingeniously utilized, the shrimps are infected by the WSSV, the shrimps use the dunaliella as bait, and the dunaliella cells belong to a green expression system and can be used for expressing and producing heterologous protein. WSSV cyst membrane protein genes, i.e. Vp19 and VP28 are obtained through the clone by a genetic engineering measure, the recombinant plasmid pBI-VP28, pBI-VP19 and pBI-VP28+VP19 are respectively built, the built recombinant plasmid is converted into the dunaliella cells, the converted dunaliella strain capable of stably expressing the virus cyst membrane protein is screened and cultured, the transgene dunaliella strain is directly fed to the shrimps as living body bait, and the immune protection effect on the shrimp bodies is improved. The prepared transgene dunaliella strain can be used for stably expressing the active protein, the protein does not need to be extracted and can be directly used as the live vaccines for feeding the shrimp bodies, the operation is convenient, the cost is low, safety and nontoxicity are realized, and the immune effect is high. |
| priorityDate | 2011-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.