http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102424847-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c827f5a27a8db928ab34128c3be46c6b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2011-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68ac34fb5fc0032930e48f4859f96102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0f08d0165f44738886d5ae35f156c02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd03db999bf3203109ada7d6ef8e8dd3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec9b12227af97c598c592bf654ca08ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eca9e538b124ddf5e580b05c026e1dbd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f0d56254eeb74c32cf95a2b8a317f86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10ecc754fe127490123293a54ba693b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a24dc101b5af1fa3e4b20b437ad00486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_964853d8906b574c60b6d76b0a1754ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4da0bb1c048b0c9b9bfa4a1865de6f90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a7372e063f09b81fb635b158abacf7b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96d72ee9ded6afaafd045e0bd2188de2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e9b17cb62aa1c89e853a728a62027e7 |
| publicationDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102424847-B |
| titleOfInvention | Bovine degenerative axonopathy carrier screening method and kit thereof |
| abstract | The invention provides a bovine degenerative axonopathy carrier screening method and a kit thereof, and belongs to the field of animal husbandry technology. According to the method, genomic DNA of bovine to be tested is used as a template; an upstream primer and a downstream primer are designed to carry out a PCR amplification reaction on the template; then a PCR amplification fragment is retrieved and digested by FastDigest restriction enzyme TspRI; and gel testing is performed after the digestion of the reaction system. Bovine to be tested which shows a digested DNA fragment of 307bp by the gel testing after digestion is normal bovine. Bovine to be tested which shows digested DNA fragments of 307bp, 225bp and 82bp after digestion is a degenerative axonopathy carrier. Bovine to be tested which shows the digested DNA fragments of 225bp and 82bp after digestion is degenerative axonopathy bovine. The screening method has advantages of simple and fast operation, low cost and high reliability. The method provided by the invention can be used to eliminate the bovine degenerative axonopathy carrier at an early stage and greatly reduce the loss of cattle industry. |
| priorityDate | 2011-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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