http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102417911-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2010-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a06494d67ca781dfac09be27a578865a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb3e3bdeaff30e133a3827b4be6ee7f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_283082ba60fb7633007d0d7a31293c0e |
| publicationDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102417911-B |
| titleOfInvention | Method for over-expressing brassica napus BnLAS gene for improving plant drought resistance |
| abstract | The invention belongs to the field of plant genetic engineering, and specifically relates to the cloning of a BnLAS gene of brassica napus with drought resistance, and the over-expression of the gene in arabidopsis thaliana. With the method, an arabidopsis thaliana transgenic plant with high drought resistance can be obtained. The method comprises steps that: primers represented by SEQ ID NO: 2 and 3 are used for amplifying a brassica napus genome DNA fragment; it is determined that the sequence of the amplified DNA fragment is a homologous sequence with that of a LAS gene in arabidopsis thaliana, and the gene is named BnLAS, wherein the nucleotide sequence of the gene is represented by SEQ ID NO: 1; RNAs of different tissue sections of brassica napus are extracted, an expression level is detected through RT-PCR, and it is found that the gene has time expression difference in brassica napus; an over-expression vector is established by using the BnLAS gene and a CaMV35S promoter, and the over-expression vector is used for transferring wild arabidopsis thaliana through an agrobacterium-mediated genetic transformation method. With the RT-PCR technology, over-expression of the BnLAS gene in a BnLAS-gene-positive transgenic plant is identified, and an arabidopsis thaliana transgenic plant with drought resistance is obtained with the cooperation of field morphological identification. |
| priorityDate | 2010-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.