http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102409065-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_62fd70cba2aa0f3d0671e6f392868dd6 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 |
| filingDate | 2011-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c970c6838bb6bd372beb65ca864c4805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1bf520cdafc3362fbc989cac8c0ceed |
| publicationDate | 2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102409065-B |
| titleOfInvention | Novel shRNA for increasing animal fertility and application thereof |
| abstract | The invention discloses novel shRNA for increasing animal fertility and an application thereof, and the shRNA is characterized in that the shRNA comprises gene sequences of Sequence NO. 15 and Sequence NO. 16. According to the invention, several pairs of shRNA single-chain oligonucleotides are designed and synthesized based on the nucleotide sequence of sheep interferon INHA gene alpha subunit; an INHA shRNA interference vector pGFP-V-RS-shRNA is constructed; the interference effect is screened by a RT-PCR method so as to obtain a pGFP-V-RS-shRNA vector with good inhibition effect; the vector comprises gene sequences of Sequence NO. 15 and Sequence NO. 16; and a shRNA lentivirus interference vector pLenti6-shRNA3 is further constructed which comprises the gene sequences of Sequence NO. 15 and Sequence NO. 16. The invention also discloses an application of the novel vector. |
| priorityDate | 2011-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 215.