http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102409061-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41d42dda56335b56f7f1545dcbc7ca22 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2011-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6fedd2201b2e44835d3ea11f06093388 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e2c9b4e17f233ce75137677a06d18f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d111cef67798f54a317848a425618bc7 |
| publicationDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102409061-B |
| titleOfInvention | Preparation method of T vector based on EGFP (enhanced green fluorescent protein) |
| abstract | The invention discloses a T vector based on EGFP (enhanced green fluorescent protein) and a preparation method thereof, relating to the field of gene engineering. The prepared T vector adopts a constitutive strong promoter to mediate expression of the EGFP gene, insertion of exogenous DNA(deoxyribonucleic acid) can block expression of the EGFP so that the positive colony is white and the false positive colony generated by vector self-linking presents green fluorescent in the sun. When the designed T vector is applied to T-A cloning, X-Gal and IPTG (isopropyl-betal-D-1-thiogalactopyranoside) are not used, thus saving the experiment cost and avoiding false positive caused by inactivation or nonuniform coating of X-Gal and IPTG. |
| priorityDate | 2011-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.