http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102408993-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_027f45fc13b9718e9ca307160c45af25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3ca8bbafc9fabbc26051d7330227a75f |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-04 |
filingDate | 2011-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c634fd8fc686fca7828328b7d5be3c93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06bfba9cfa7f00bba7863d933b558cf5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ec4d0fa2214de1925c236bed2cc2699 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11b1340aba5a43ea1decf981c0333810 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a738bf30b53382d79d3b32f40d907d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c46c9d4e14049cf1abda75c9ee41f2d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16800c46b85b59098e68ac4140907470 |
publicationDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102408993-B |
titleOfInvention | Bifidobacterium bifidum anti-freeze culture medium and application method thereof |
abstract | The invention provides a bifidobacterium bifidum anti-freeze culture medium, which includes the following components by weight: 16-20 g of lactose, 3-5 g of yeast extract, 6-9 g of sodium chloride, 0.6-1.0 g of sorbitol, 0.2-0.5 g of glutamic acid, 8-12 g of soybean peptone, 0.5-2.0 g of diammonium hydrogen citrate, 0.2-0.5 g of betaine, 1.0-2.5 g of dipotassium phosphate, 0.4-0.8 g of sodium ascorbate, 0.1-0.2 g of MgSO4, 0.5-1.0 ml of Tween-80, 3-5 g of cysteine hydrochloride and 1,000 ml of distilled water. In the bifidobacterium bifidum anti-freeze fungal powder is prepared by using the anti-freeze culture medium, the freeze-dry survival rate is over 58 percent, and viable count of fungal powder is over 2.0*10<11> cfu/g; the freeze-dry survival rate of the fungal powder prepared by MRS (de Man, Rogosa and Sharp) broth is 8.74 percent at most; and the bifidobacterium bifidum anti-freeze culture medium does not contain dried skim milk or trehalose, so that the problem of high probability of water absorption and moistening in storage of the fungal powder is solved. |
priorityDate | 2011-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 80.