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filingDate 2011-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c634fd8fc686fca7828328b7d5be3c93
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publicationDate 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102408993-B
titleOfInvention Bifidobacterium bifidum anti-freeze culture medium and application method thereof
abstract The invention provides a bifidobacterium bifidum anti-freeze culture medium, which includes the following components by weight: 16-20 g of lactose, 3-5 g of yeast extract, 6-9 g of sodium chloride, 0.6-1.0 g of sorbitol, 0.2-0.5 g of glutamic acid, 8-12 g of soybean peptone, 0.5-2.0 g of diammonium hydrogen citrate, 0.2-0.5 g of betaine, 1.0-2.5 g of dipotassium phosphate, 0.4-0.8 g of sodium ascorbate, 0.1-0.2 g of MgSO4, 0.5-1.0 ml of Tween-80, 3-5 g of cysteine hydrochloride and 1,000 ml of distilled water. In the bifidobacterium bifidum anti-freeze fungal powder is prepared by using the anti-freeze culture medium, the freeze-dry survival rate is over 58 percent, and viable count of fungal powder is over 2.0*10<11> cfu/g; the freeze-dry survival rate of the fungal powder prepared by MRS (de Man, Rogosa and Sharp) broth is 8.74 percent at most; and the bifidobacterium bifidum anti-freeze culture medium does not contain dried skim milk or trehalose, so that the problem of high probability of water absorption and moistening in storage of the fungal powder is solved.
priorityDate 2011-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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