http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102401830-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c2dab8468bb86892bd9b3ce8735d66e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532 |
| filingDate | 2011-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f703697603295afe7ff01aa9a474a89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11380b9ee0a2225c438e26bccc539284 |
| publicationDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102401830-B |
| titleOfInvention | Enzyme-linked immunoassay method for food preservative nisin |
| abstract | The present invention provides an enzyme-linked immunoassay method for a food preservative nisin. According to the present invention, the nisin is adopted as antigen to carry out animal immunization to obtain polyclonal antibody; a conjugate of the nisin hapten and ovalbumin (OVA) is adopted as coating antigen; the nisin is adopted as a standard substance; a treatment method for food samples by the immunoassay is established; a carrier kit of the method is provided. The establishment of the immunoassay method comprises: treating the sample requiring detection, preparing the coating antigen, preparing the polyclonal antibody, carrying out a competing reaction, adding enzyme-labeled second antibody, carrying out a color reaction, and the like. The obtained immunoassay kit comprises: an antigen-coated enzyme label plate, a bottle of an enzyme-labeled second antibody working solution, a bottle of an antibody working solution, six bottles of nisin standard substance solutions, two bottles of substrate coloration solutions, a bottle of a stop buffer, a bottle of a concentrated cleaning solution and a bottle of a reconstituted solution. |
| priorityDate | 2011-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.