http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102382890-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6474bcaf9f7b4f98e4f9643e3f3cd606 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2011-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_386b412c445fd848fd458582ee31de87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47b35d4c9fc528ff28632086460750d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99d3adff8ff8ed42cc774ee125edac9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfe487cb938c734558757880fc3a95ac |
| publicationDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102382890-B |
| titleOfInvention | Pythium splendens braun fluorescence quantitative PCR detection reagent and detection kit and application |
| abstract | The invention discloses a pythium splendens braun fluorescence quantitative PCR detection reagent, a detection kit and an application; the detection reagent comprises a pair of specific primers with sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2, and a specific fluorescence probe with a sequence as shown in SEQ ID NO: 3; an amplification target fragment has a length of 94 bp, and the nucleotide sequence of the amplification target fragment is shown in SEQ ID NO: 4; the detection kit comprises components of a CTAB extract, a PCR buffer containing the primers and the probe, TaqDNA polymerase, positive control liquid, and quantitative standard liquid; the detection method comprises the extraction of total RNA and the fluorescence PCR reaction; detection performed by using the pythium splendens braun fluorescence quantitative PCR detection reagent overcomes disadvantages of time consumption, easy pollution, and electrophoresis detection necessity after amplification of routine PCR, can realize rapid and accurate qualitative and quantitative detection of pythium splendens braun in a sample, and has the advantages of simplicity, easy operations, intuitive results, high sensitivity, good repeatability, and the like. |
| priorityDate | 2011-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.