| abstract |
The invention relates to the field of animal disease molecular biology detection methods and detection agents, in particular to an animal Torque Teno virus rapid detection primer, a kit and a detection method using a loop-mediated isothermal amplification (LAMP) technology. Multiple comparisons are carried out by using Clustal W according to two different serotype gene sequences of an animal Torque Teno virus, and conserved zones of the sequences are analyzed, an inner primer and an outer primer are respectively designed by adopting LAMP primer design software, and detection and identification of TTV1 and TTV2 serotypes are realized rapidly specifically. The animal Torque Teno virus detection method disclosed by the invention has the advantages of rapidness, high sensitivity, strong specificity, convenience for operation, low requirement of equipment, time and labor saving, easiness in observing results, no complex post-treatment, and wide application range. |