http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102353730-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
filingDate | 2011-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b32a76ad239b7b81d0403ce3efc6aecf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a8e66c20dc995e64127f13f99b92ebc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96d45a7daf23032ce557daca14bd9cf5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a6b6d18b7d4f51a52192deab7363109 |
publicationDate | 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102353730-B |
titleOfInvention | Detection method for galacto-oligosaccharide based on biological enzyme technology |
abstract | The invention provides a detection method for galacto-oligosaccharide based on biological enzyme technology, which belongs to the technical field of food engineering. According to the invention, at first, the method of removing proteins by using trichloroacetic acid and lead acetate is employed for pretreatment of an object to be detected, and membrane filtration is utilized to remove impurities so as to obtain a sample to be detected; then, sodium hydroxide and sodium acetate with different concentration are utilized for ion exchange and gradient elution; finally, a four-potential pulsed amperometric detector is used for detection. The invention is applicable to separation and detection of cow's milk containing galacto-oligosaccharide and a variety of galacto-oligosaccharide components in a galacto-oligosaccharide solution system; employment of ion chromatography, utilization of the methods of ion exchange and gradient elution for separation of galacto-oligosaccharide and cooperative detection with the four-potential pulsed amperometric detector enable contamination and interference to an analytical column, the detector and the like caused by macro-molecular substances such as proteins, fat and emulsifiers which might exist in the sample to be eliminated, thereby allowing accurate and rapid detection to be achieved. |
priorityDate | 2011-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 153.