http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102353713-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cb011d981829bdcc85a40133a181da8 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-48 |
filingDate | 2011-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7985c1dd581b40435f210af639dea5e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_533dca1c8deeafbe148e604cac3ffafb |
publicationDate | 2014-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102353713-B |
titleOfInvention | Detection methods for activity and substrate concentration of oxidase |
abstract | The invention provides a detection method for activity of oxidase. The method comprises the following steps: mixing a solution to be detected with an oxidase substrate and arylborane compounds so as to obtain an intermediate solution to be detected containing phenolic compounds; detecting the concentration of the phenolic compounds in the intermediate solution to be detected by using an electrochemical detection method and calculating the activity of oxidase in the solution to be detected according to the concentration of the phenolic compounds. According to the invention, the activity of oxidase is indirectly detected based on electrochemical detection of the concentration of the phenolic compounds; the phenolic compounds have the characteristic of low potential, so it is easy to carry out electrochemical detection; since the electrochemical detection method has the characteristics of simple operation and high sensitivity, the detection method provided in the invention has the characteristics of simple operation and high sensitivity and detection accuracy. The invention also provides three detection methods for substrate concentration of oxidase. The detection approach of the three detection methods is identical with that of the above-mentioned detection method, and the detection methods have the characteristics of simple operation and high sensitivity and detection accuracy. |
priorityDate | 2011-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.