http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102352409-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d79709ccbb7c21cbb6a0007d1d28494f |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2011-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_971fc65c7f7690b82cf3a84a7d7eadc9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f92c8b42f6d0709eb5507da62e4795d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a59cf1533e447cf491955612a9489df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85a5d0b2e4dfd1169db839fafe801a41 |
| publicationDate | 2014-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102352409-B |
| titleOfInvention | Method and kit for gene sequencing and typing of human major histocompatibility complex class I chain related gene A (MICA) |
| abstract | The invention relates to a method and a kit for gene sequencing and typing of a human major histocompatibility complex class I chain related gene A (MICA). The method comprises the following specific steps: extracting sample genome DNA (deoxyribonucleic acid); designing a PCR (polymerase chain reaction) primer and carrying out PCR amplification on the sample genome DNA; designing a sequencing primer, and carrying out dual-direction sequencing on the obtained PCR amplification product, namely respectively carrying out forward and reverse dual-direction sequencing on 1,2,3,4,5 and 6 exons in the PCR amplification product; and typing according to the sequencing result. By using the method, the problem that the existing imported MICA commercialized sequencing kit is just a single-direction sequencing kit is solved, the defects that the base signal intensity of single-direction sequencing is not in consistence and the existing of mutation can be not clearly reflected are avoided, ambiguous gene typing result of MIAC is solved, and the base sequences of the first and sixth exons are obtained. |
| priorityDate | 2011-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.