http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102323424-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-536 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2011-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d2523ec84344178b8386661ba183105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcd1a301b5c5a948f6f360c566542f9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cec2841c9aab39a89a6e2b6482a17962 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f0422e2c76534b4e7eeb3e8c2dc8410 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2125579013525cd75e5d26c5a17c0b3 |
publicationDate | 2014-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102323424-B |
titleOfInvention | In-situ detection reagent and method for exogenous biomolecules on surface of environmental particulate matter |
abstract | The invention discloses an in-situ detection reagent and method for exogenous biomolecules on the surface of an environmental particulate matter. Aiming at detecting an insecticidal protein CrylAb absorbed on the surface of a soil particulate matter, the surface of a magnetic fluorescent nano/micro-sphere is connected with a CrylAb protein antibody; after soil particulate matter suspension and the magnetic flourescent nano/micro-sphere are hatched for a period of time, the soil particulate matter with the surface absorbing CrylAb protein captures the magnetic flourescent nano/micro-sphere through the interaction of antigen antibodies; and after magnetic separation, the particulate matter suspension with magnetism is placed in a quartz cuvette, and a fluorescence signal generated by the particulate matter is detected by using a fluorescence spectrophotometer. The fluorescence intensity of the fluorescence signal is proportional to the CrylAb protein absorbed on the surface of the soil particulate matter within a certain range, therefore, the CrylAb protein absorbed on the surface of the soil particulate matter can be directly quantified. Or dynamic quantitative analysis can be carried out by adopting a fluorescence microscope and a laser induced fluorescent scattering pulse spectrum. |
priorityDate | 2011-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.