http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102321666-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 |
filingDate | 2011-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4891d7bb595215ab654fca0688779f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4509940604e04cb01bc1ffe64fe7601 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d83b9a005564953885855cb65d0a0843 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45fb0653a11fd0c25b5d3ff02538b3e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f9d596f38bc43027cf12c5731172bf6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18e028e0873f09e7917b7070fb86bb84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88d22e147732306a644a4eddc8f47479 |
publicationDate | 2013-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102321666-B |
titleOfInvention | Rapid preparation method of dicotyledonous plant transgenic acceptor |
abstract | The invention discloses a rapid preparation method of a dicotyledonous plant transgenic acceptor, which comprises the following steps: 1) surface sterilization of dicotyledonous plant seeds; 2) imbibition of the dicotyledonous plant seeds, that is, putting the sterilized dicotyledonous plant seeds into a sterilized differentiation tank, adding sterile water, soaking the sterilized dicotyledonous plant seeds in the dark at 18-22 DEG C for 12-15 hours to obtain the imbibed dicotyledonous plant seeds; 3) acceptor preparation, that is, putting the imbibed dicotyledonous plant seeds on sterile absorbent paper, cutting the seeds vertically along the hila to divide the cotyledon into two petals, removing seed coats to obtain the dicotyledonous plant transgenic acceptors. With the invention, the transgenic program is simple and practical, has stable efficiency, and has great significance for the improvement of dicotyledonous plant transgene efficiency. |
priorityDate | 2011-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.