http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102311970-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2011-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_292765e82f62bc02ec9527c7eeba74df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a694fd3b29a9e75206e42ed064aeb91f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_578bf84672de8d61a7fc926fadf9006a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58091d57cae77ca5138698ab7f57d6c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ed62af961d1f9d4e650cc7c610d1d7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bacd2d108634b64b0cd5e16c17deabc7 |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102311970-B |
| titleOfInvention | Expression cassette mazF-cassette for expressing escherichia coli toxin protein mazF gene and construction method and application thereof |
| abstract | The invention discloses an expression cassette mazF-cassette for expressing escherichia coli toxin protein mazF gene, the nucleotide sequence of which is SEQ ID NO:1. The invention also discloses a construction method of the expression cassette mazF-cassette, comprising the following steps of: 1) amplifying the escherichia coli toxin protein mazF gene; 2) amplifying xylose-induced promoter Pxyl A gene; 3) amplifying a resistant gene zeocin; 4) fusing the above three PCR segments into a recombinant PCR segment; 5) carrying out enzyme digestion on the recombinant PCR segment by the use of restriction enzyme Sal I/Xba I, connecting with an integrative vector Psg1729 which undergoes the same enzyme digestion, transforming bacillus subtilis 1A751 to obtain a transformant which is a host bacteria containing the expression cassette. The expression cassette mazF-cassette can be used to construct a bacillus subtilis general label-free homologous recombination system. |
| priorityDate | 2011-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.