http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102304160-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ef0c06d538a4a90a27d36888e71a1670 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K36-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P9-10 |
| filingDate | 2011-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8c6bbca41016119de1f0531d71a70b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ecac5e8a941bcb18e6d14f94966f5ea6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_781355128a6a8ec651733225aef5b4db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83bf0f1f7639a0aa74a14d260c280681 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d09023da2f543db77dbcf1ebb8280ae |
| publicationDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102304160-B |
| titleOfInvention | Preparation method and application of luteolin-7-O-gentibioside |
| abstract | The invention discloses a method for preparing luteolin-7-O-gentibioside, which comprises the following process steps of: adding water to decoct a medicinal material of herb of sowthistleleaf ixeris twice, 1 hour for the first time and 0.5 hour for the second time, mixing decoction, concentrating until 1ml of decoction is equivalent to 0.5g of original raw medicine, naturally cooling to the temperature of below 40DEG C, adding 95 percent ethanol in an amount which is 2 to 4 times weight of the concentrated decoction with stirring to make the alcohol content is 50 to 70 percent, standing for 24 hours, and filtering; recovering ethanol from filtrate until alcohol smell is absent, making recovered liquid pass through a macroporous resin column, eluting 1 to 5 bed volume (BV) by using water, discarding aqueous eluant, eluting 5 to 10VB by using 30 percent ethanol, collecting eluant, and concentrating under reduced pressure; loading the concentrated solution on a polyamide column by using a dry process, eluting 5 to 10BV by using water, discarding aqueous eluant, eluting 5BV by using 30 percent ethanol, collecting 2 to 5BV of eluant, and concentrating under reduced pressure until dryness; and dissolving by using methanol, and performing high performance liquid chromatography (HPLC) separation preparation by applying 250*10mm semi-preparation column and taking acetonitrile and 1 percent glacial acetic acid in a ratio of 15:85 as mobile phases to obtain yellow amorphous powder. The process is simple and the purity of the product is over 98 percent. |
| priorityDate | 2011-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.