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grantDate 2014-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2014-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102302539-B
titleOfInvention Method for producing trifolium pratense L. isoflavones
abstract The invention relates to a method for producing trifolium pratense L. isoflavones, which is characterized by comprising the following steps of: a, extracting, namely extracting trifolium pratense L. by using methanol; b, filtering, namely standing extract in a storage tank, and filtering to remove slag; c, decolorizing and concentrating, namely decolorizing by using an activated carbon column, concentrating, extracting by using petroleum ether and ethyl acetate respectively, and concentrating to obtain a dry product; d, regulating the ph value, namely dissolving the dry product in NaOH aqueous solution, and controlling the ph value to be between 7 and 9; e, loading to a column, namely loading AB-8 macroporous adsorption resin to the column, eluting by using methanol solution, and evaporating until solution is dried; and f, extracting and crystallizing, namely dissolving the dry product in the NaOH aqueous solution, extracting by using the ethyl acetate, concentrating to reach certain volume, and performing cooling crystallization. An isoflavone product with high purity is obtained through steps of extracting solution and column chromatography solution recycling, two-time extraction, column chromatography and the like; a large quantity of solvent, electricity and heat are saved, and the service cycle of resin is prolonged; and by the process, product quality is improved, and energy consumption is reduced.
priorityDate 2010-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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