http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102297905-B

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-08
filingDate 2010-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db4f66c84d1f4f310c109b56e2c5bfb9
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publicationDate 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102297905-B
titleOfInvention HPLC (High Performance Liquid Chromatography) method for utilizing chiral column to separate, identify and prepare monomer matter from cucurbitacin mixture
abstract The invention discloses an HPLC (High Performance Liquid Chromatography) method for utilizing a chiral column to separate, identify and prepare a monomer matter from a cucurbitacin mixture. The process conditions of the HPLC method are as follows: the chiral column using an amylose derivative as a solid phase is taken as a chromatographic column; more than two mixtures of isopropanol, alcohol, methyl alcohol, acetonitrile, normal hexane, isohexane, n-pentane, trifluoroacetic acid and glacial acetic acid are taken as mobile phases; and a detection wavelength is 220nm-270nm. The HPLC method provided by the invention is characterized by simple operation, high accuracy and strong specificity. The HPLC method can be used for excellently separating matters, such as cucurbitacin B, iso-cucurbitacin B, dihydro cucurbitacin B, cucurbitacin E, and the like, from the cucurbitacin mixture, can be used for preparing various cucurbitacin monomers from the cucurbitacin mixture and also can be used for accurately measuring contents of cucurbitacin raw materials and various cucurbitacins in a prepared product.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108593787-A
priorityDate 2010-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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