http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102293154-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1757bfe8a478e7820914b0992e8fd9f1 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2011-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89e09c01a567c51ac7163f72caaf5ed6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_521b00bcb5d8a802e80833a5f7a17599 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1f992fed8db5738337ee0e0b085c5a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_020dbca4562e5965c21601ccf89e2f56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c639680d90272a128decdf7e3e597e2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_625245347017d52308b1ab95915cab33 |
| publicationDate | 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102293154-B |
| titleOfInvention | Mutagenesis method of nicotiana tabacum L. pollen |
| abstract | The invention relates to a high-efficiency mutagenesis method of nicotiana tabacum L. pollen. The high-efficiency mutagenesis method comprises the following steps of putting nicotiana tabacum L. pollen into a liquid medium under an aseptic condition to carry out mutagenesis treatment by 0.1 to 0.2% of ethyl methanesulfonate (EMS) in a dark place at a temperature of 30 to 32 DEG C, carrying out chromosome doubling treatment by a NLN-16 liquid medium in a dark environment at a temperature of 30 to 32 DEG C, putting the nicotiana tabacum L. pollen obtained by the previous step into a NLN-13 liquid medium to carry out embryo induction culture in a dark environment at a temperature of 24 to 26 DEG C, carrying out shake culture by a shaking table in a dark environment at a temperature of 24 to 26 DEG C when an immature embryo appears, and putting the immature embryo growing a cotyledon into a solid medium to carry out subculture, wherein during a transplanting season, after seedling hardening, a seedling obtained by the high-efficiency mutagenesis method can be transplanted in a field for mutant character identification. The high-efficiency mutagenesis method overcomes dominant and recessive barriers of gene expression, is beneficial for selection, shortens greatly a mutant character stabilization period, saves breeding generation time, and has the characteristics of wide variation range, high mutagenesis frequency and the like. |
| priorityDate | 2010-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.