http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102283119-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15d96294a08120ddf37c00df24cf3450 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2011-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d5b2f034e8002ff3bdb6f1e5f793e5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e652c8d95bc5c24921f5bef9eaa8023b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3ac5b4475b59c9c40933fbb478eed29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c6d034c95ba340980d0d622376284aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06f33d188e96d2004baa39cc301b5a09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcd1e52353d78c1c95c306a5c5cbb290 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_077f67eaa38a194cacb41d9d1daad46b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_605d78c1d11590f0a693ec24e6855585 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d39ec95b5ca7a8c1390636787c2433d8 |
| publicationDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102283119-B |
| titleOfInvention | Method for preparing sterile rose vine for tissue culture |
| abstract | The invention relates to a rose tissue culture technique and aims to provide a method for preparing a sterile rose vine for tissue culture. The method comprises: trimming and washing a rose vine, soaking the rose vine in phenylthiocarbamide (PTC) aqueous solution at a concentration of 25 percent (v/v), and washing with distilled water; filling the rose vine forward and vertically into a tissue culture bottle which is sterilized by high-pressure steam, covering the bottle cap, placing the bottle in a 37 DEG C constant-temperature tank for 24 hours, transferring the bottle to a 4 DEG C refrigerator for 48 hours and repeating the step once; and cutting the rose vine into small sections with axillary buds under a sterile condition, sterilizing by soaking and in 70-percent alcohol and by shaking, taking the small sections out, washing the small sections with sterile water, soaking in 0.002 liter of mercury for 9 minutes, taking the small sections out, washing with sterile water, and obtaining the sterile rose vine for tissue culture. With the PTC aqueous solution and alternating treatment at 37 DEG C and 4 DEG C, the method has the advantages of higher sterile effect, higher germination rate, simpler and more convenient operation, time and labor conservation, lower cost, higher production application performance and the like compared with the conventional method. |
| priorityDate | 2011-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.