| abstract |
The invention discloses a fusion protein of hepatic-targeted peptide and human interferon a2b, and a preparation method and application thereof. The nucleotide sequence of the fusion protein of hepatic-targeted peptide and human interferon a2b provided in the invention is described by SEQ ID NO. 1, and the amino acid sequence coded by the nucleotide sequence is described by SEQ ID NO. 2. According to in vitro tests, the fusion protein provided in the invention has obvious activity of resisting HBV and hepatocyte targeting. Since characteristics of both hepatic-targeted peptide of plasmodium circumsporozoite protein CSPI-plus and human IFNa2b are combined into the fusion protein in the invention, the fusion protein can be used for preparation of novel hepatic-targeted antiviral drugs and is of profound significance for lowering down morbidity and mortality of liver diseases caused by infection of HBV. According to the invention, the prepared fusion protein has a high expression level and is easy to purify, production period for the fusion protein is short, and low cost is realized; therefore, the invention has important application prospects and is of practical significance. |