http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102266589-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eda37409f26cb8e833ba67f2b1bc1fd5 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J3-075 |
| filingDate | 2011-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0c43d8b999e20f9aea93f9f3cc8e4f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08fed353c06dc9c6149ad93c3768188a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8876f3550fe771889a99c857e4ce175d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e562258403612aea72972f1ee7d06814 |
| publicationDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102266589-B |
| titleOfInvention | Preparation method of mechanically-enhanced cell-loaded microchannel hydrogel |
| abstract | The invention relates to a preparation method of a mechanically-enhanced cell-loaded microchannel hydrogel, which comprises the following steps: preparing a mixed solution of hydrogel sol and cell culture fluid, and pouring the sol-cell mixed liquid into a polymethyl methacrylate die into which capillary tubes or fibers are inserted, thereby enabling primary crosslinking; drawing out the capillary tubes or fibers, introducing the hydrogel sol into microchannels of the cell-loaded hydrogel, and reinserting the capillary tubes or fibers, or sucking excess hydrogel sol out of the microchannels and carrying out ultraviolet irradiation, thereby enabling secondary crosslinking, so that the cell-loaded hydrogel with thick hydrogel layer is formed in the wall regions of the microchannels; and drawing out the capillary tubes or fibers, introducing endothelial cell culture fluid into the microchannels of the cell-loaded hydrogel, and culturing so that the endothelial cells grow and spread to form endothelial microchannels. The introduction of the thick hydrogel layer can maintain the stability of the microchannels in the hydrogel, and prevent shedding and intrusion of the endothelial cells;and the culture fluid in the microchannels can be filtered and screened. |
| priorityDate | 2011-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.