http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102253207-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db3ac4ed81f0b1ac6f0f1beee265b9ad |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 |
| filingDate | 2011-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0e40303694c31566e12f65ff027d5cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_043f0608a26a5d56c20080403921010c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdbce97b1e2329b5ac37ee14f51a64d5 |
| publicationDate | 2014-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102253207-B |
| titleOfInvention | Method for detecting feed xylanase activity and application thereof |
| abstract | The invention discloses a method for detecting feed xylanase activity and application thereof. The method disclosed by the invention comprises the following steps: detecting the absorbance value of residual biotin labeled xylan in the sample to be detected after the xylanase hydrolysis by using ELSA (enzyme linked immunosorbent assay); and calculating the activity of xylanase in the sample to be detected according to an enzyme activity gradient curve, wherein the enzyme activity gradient curve is a curve of the corresponding relationship between the absorbance value of residual biotin labeled xylan after enzymolysis detected by ELSA and the known different xylanase activities. As the ELSA conditions are optimized in the invention, the method disclosed by the invention can perform trace detection on the xylanase activity in feed, and can realize sensitivity of 1*10<5>U/g. The method disclosed by the invention not only can detect activity of xylanase in feed, but also enables the enzymolysis temperature adopted for detection to be consistent with the temperature of intestinal canal of livestock/poultry due to high sensitivity; and thus, by adopting the method to obtain the enzyme activity, better activity evaluation can be performed on the xylanase in feed. |
| priorityDate | 2011-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.