http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102234686-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3d4964b5a4fef7e756d169c4a0b9c75 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2010-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dfe199e3a7de11cc9b11008e72a72e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27cd769b6c43f11d1b233c1f38a7656b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1299c8d600bfc237fbb8c8e47fe92860 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f39af7f39ddd719a622b4991758f5f5 |
| publicationDate | 2013-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102234686-B |
| titleOfInvention | Liquid chip for detecting KRAS gene mutation |
| abstract | The invention discloses a liquid chip for detecting KRAS gene mutation. The liquid chip mainly comprises: (A) respectively designed wild type and mutant ASPE primer pairs aimed at KRAS gene mutation sites; (B) microspheres respectively coated with specific anti-tag sequences, wherein the microspheres have different color codes, and the anti-tag sequences can be complementarily coupled with selected tag sequences; (C) amplimers used for producing KRAS gene target sequences containing corresponding mutation sites of Codon 12, Codon 13 and/or Codon 61 through amplification. The result obtained with the detection method provided by the present invention has a coincidence rate reaching 100% with that of a sequencing method. The prepared liquid chip for detecting KRAS gene mutation has excellent signal to noise ratio. Basically no cross reaction occurs between designed probes and the anti-tag sequences. With the liquid chip provided by the present invention, parallel detections of various mutants on a same site and various mutation sites can be realized. |
| priorityDate | 2010-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.