http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102226779-B

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filingDate 2011-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d3ac696eacca08ac2ed49f223e6207d
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publicationDate 2013-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102226779-B
titleOfInvention Electrochemical immunodetection method
abstract The invention discloses an electrochemical immunodetection method based on a thionine-graphene nano composite double coated with nano platinum chitosan. According to the invention, double antibody sandwiched method is adopted, an abnormal prothrombin(APT) antibody supported on the surface of electrodes is subject to an immunization reaction with an abnormal prothrombin(APT) in a sample solution, then combines with the coupling object of the thionine-graphene nano composite double coated with nano platinum chitosan and the abnormal prothrombin(APT). Based on the electrochemical activity of thethionine-graphene nano composite double coated with nano platinum chitosan, peak current value of reduction peak of cyclic voltammetry is detected so as to further detect the concentration of the abnormal prothrombin in the sample under test. The method has the linear response range of 0.8-800ng/ml, the lower limit of the detection of 0.23ng/ml, with good specificity and high sensitivity, and is significant to the diagnosis of the abnormal prothrombin(APT).
priorityDate 2011-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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