http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102221610-B

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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
filingDate 2010-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5392019dc24bd0e7474abd9bc53af45
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publicationDate 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102221610-B
titleOfInvention Method for rapidly detecting pathogenic bacteria at high efficiency, biological dependent sensor and preparation method thereof
abstract The invention provides a method for rapidly detecting pathogenic bacteria at high efficiency, a biological dependent sensor and a preparation method thereof, wherein the biological dependent sensor comprises a plastid with the function of converting signals and a capturing system with the function of recognizing molecules; the method for rapidly detecting the pathogenic bacteria at high efficiency comprises the following steps: preparing the biological dependent sensor; using the biological dependent sensor to capture a sample detecting solution; simultaneously setting the blank comparison and the positive concentration comparison of a standard substance and starting anadenosine triphosphate synthetic reaction; and carrying out the detection by utilizing a fluorescence scanner. The scheme in the invention has the advantages of low detection limit and short detection time; the detection limit can reach 100cfu/hole; if the detection limit is computed according to 1cfu/25g, the whole detecting course is not beyond 12 hours, is shortened by at least 60 hours than the conventional microorganism test method and is shortened by at least 36 hours than a PCR (Polymerase chain Reaction) method; and when the concentration of the bacteria in a sample is higher than 1cfu/25g, the needed time is short, thus the deep influence on food safety and disease treatment bacterium control can be generated.
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