http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102220272-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_01b63086f0a0411f410da44b3b3a847e |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-21 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2011-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0159d1715a34c1b6586ddf498a81d64c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e8f46f861380768d5516d80ff29cad3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb37d46ef6c32630248aead6acf7fa21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f7abefe0be1ffaca1eaf43cad68a41d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2eb567699eccd8cbdbfdb6ebf3143fb5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d333fa17b712d9fd2521dacedcb0c42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c124b968d05c0829a1ca9e24d2d5dce8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_375f354cf506df94b5c4d88718963b34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9bff36e43e8c7a01f66a64007ed7759 |
| publicationDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102220272-B |
| titleOfInvention | Method for high density culture of haemophilus parasuis for preparing vaccines |
| abstract | The invention discloses a method for the high density culture of haemophilus parasuis for preparing vaccines, comprising the following steps: A, flat-activating freeze-dried seed strain 4, 5 and (or) 13 by using TSA for 10-16h until single colonies grow; B, selecting 3-6 single colonies to put in a TSB shake-flask first seed culture medium, cultivating for 8-14h until OD600 value reaches 0.4-0.6; C, transferring the first seed liquid into a second shake-flask culture medium with the inoculation amount of 1-5% V/V, cultivating for 8-12h up to exponential phase; and D, transferring the second seed liquid into a fermentation-cylinder fermentation medium with the inoculation amount of 1-3% V/V, controlling PH in 6.8-7.2 and OD in 40-60% to maintain the microbe logarithmic growth, when PH value becomes stable and dissolved oxygen rises, taking a pot to collect the bacteria to obtain the somatic antigen of haemophilus parasuis with high density. The method has the advantages of wide raw material source, lost cost, fast microbe growth and high density, and is easy to realize the large-scale high density culture of somatic antigen of haemophilus parasuis. |
| priorityDate | 2011-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.