http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102212602-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P23-00 |
| filingDate | 2011-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_600286359d79bb753f34fb0400332336 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15cacb01ba3d7e830129b9de1fbf6306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65ed3943f2250ebb8b6ea135fd914f50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48ca95ee2ff2ec432633e1799de3b074 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb669b11b622c0371a9350f44e1f54b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30f7731a79394f09d2bc76e76cb53843 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c8d77c1e86ffc516b3203ccf9204186f |
| publicationDate | 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102212602-B |
| titleOfInvention | Method for synthesizing vitamin A lactate by catalysis of yeast display lipase |
| abstract | The invention discloses a method for synthesizing vitamin A lactate by catalysis of yeast display lipase. The method comprises the following steps of: dissolving vitamin A acetate and lactic acid into an organic solvent, adding the yeast display lipase, reacting for 10 to 14 hours in the absence of oxygen at the temperature of between 50 and 60 DEG C, and performing separation and purification to obtain the vitamin A lactate, wherein the yeast display lipase is prepared by the following steps of: transferring linearly treated recombinant plasmids to pichia pastoris GS115, inoculating the obtained transformant to a buffered methanol complex (BMMY) medium, performing induced culture for 72 to 144 hours, performing centrifugal collection on bacteria, and performing flushing, biological imprinting and freeze drying on the bacteria to obtain the yeast display lipase. The lipase is displayed outside cells, and the vitamin A lactate is synthesized by catalysis of the enzyme preparation, so that the transformation efficiency is improved, the reaction time is shortened and the production cost is reduced. |
| priorityDate | 2011-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 104.