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filingDate 2011-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48ca95ee2ff2ec432633e1799de3b074
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publicationDate 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102212583-B
titleOfInvention Method for catalyzing and synthesizing octenyl succinic anhydride modified starch ester through yeast show lipase
abstract The invention discloses a method for catalyzing and synthesizing octenyl succinic anhydride modified starch ester through yeast show lipase, comprising the following steps of: dissolving grain starch in water; after imbibition, adding octenyl succinic anhydride and the yeast show lipase; stirring and reacting at 34-36 degrees centigrade for 1-1.5 h; and separating and purifying to obtain starch sodium ester sodium octenyl succinate. A method for preparing the yeast show lipase comprises the following steps of: transforming linearly treated recombinant plasmids into pichia pastoris GS115; inoculating the obtained transformant into a BMMY (buffered methanol-complex medium) culture medium; after inducing and culturing for 72-144 h, centrifugally collecting thallus; and washing, biologically imprinting, freezing and drying the thallus so as to obtain the yeast show lipase. By showing the lipase outside cells, the octenyl succinic anhydride modified starch ester is catalyzed and synthesized through the lipase preparation. By means of the method disclosed by the invention, the transformation efficiency is improved; furthermore, the reaction time is shortened; and the production cost is reduced.
priorityDate 2011-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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