http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102212545-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee265148b3a7a38ea76bf89e12922ed3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4717 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0276 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-877 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 |
| filingDate | 2011-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2028523bcebd8d6c04bc0bfdcd7f5b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1a17b2e6f1a29de77316ecd8bf4a581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17681791db84350645819f70e4d28006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a56b75458ba478338f52b09467c998c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43e894efd5a270e355892e42c3e602f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06667daa5950db382f3fc8c8d4b82f70 |
| publicationDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102212545-B |
| titleOfInvention | Method for knocking out cattle beta-lactoglobulin gene by using zinc finger nucleases (ZFNs) |
| abstract | The invention provides a method for knocking out a cattle beta-lactoglobulin gene by using zinc finger nucleases (ZFNs), comprising the following steps of: according to a cattle beta-lactoglobulin gene sequence, designing ZFNs specific site expression vector and transplanting the ZFNs specific site expression vector into cattle fibroblast; and obtaining cells with the beta-lactoglobulin gene knocked out. By using ZFNs mediated gene knockout, one-time transfection can be realized so as to obtain cell clones with biallelic genes knocked out, which is difficultly achieved in the conventional gene targeting process. The drug screening process is saved. The method disclosed by the invention is advantageous for forming monoclonal cells. The process required by cells for resisting drug toxic process is avoided. The method plays a key role in the improvement of subsequent somatic cell nuclear transplantation efficiency and embryonic development quality. Simultaneously, resistance genes are not contained; and the biological safety evaluation process is greatly simplified. |
| priorityDate | 2011-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.