http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102212466-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d80979b7fced042bd9e177402d02731 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 |
| filingDate | 2011-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba0ffdab2d82a45cd3aa4536af990874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2077751f61e05666c5ee473e08ff01c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1953e322b1ddde4609ae69786287fc40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91eff9e8d361e73a08abc08bb28affff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_331d7441bd5e1a7a13ca5a546a58a3df |
| publicationDate | 2013-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102212466-B |
| titleOfInvention | Alkaline lysis system for preparing plasmid DNA and combined system |
| abstract | The invention provides an alkaline lysis system for preparing plasmid DNA and a combined system. The alkaline lysis system for preparing plasmid DNA contains a bacterial suspension storage tank, an alkaline lysate storage tank and a hollow ultrafiltration column, wherein the hollow ultrafiltration column contains a first cavity and a second cavity which are divided by an ultrafiltration membrane; the alkaline lysate storage tank is communicated with the first cavity; and the bacterial suspension storage tank is communicated with the second cavity. The alkaline lysis system provided by the invention adopts the hollow ultrafiltration column to mix bacterial suspension and alkaline lysate evenly and avoid the problems that the pH value is too high locally and the shear stress is too large owning to the conventional mixing method such as stirring; and the invention provides the alkaline lysis system for preparing plasmid DNA, and the system has good stability, high efficiency and high repeatability. Meanwhile, the plasmid DNA prepared by the alkaline lysis system has higher supercoiled DNA content which is up to more than 90%. |
| priorityDate | 2011-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 21.