http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102206688-B

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filingDate 2011-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48ca95ee2ff2ec432633e1799de3b074
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publicationDate 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102206688-B
titleOfInvention Method for catalytically synthesizing conjugated linoleic acid by saccharomyces cerevisiae display linoleic acid isomerase
abstract The invention discloses a method for catalytically synthesizing conjugated linoleic acid by saccharomyces cerevisiae display linoleic acid isomerase, which comprises the following steps of: inoculating saccharomyces cerevisiae K601 containing recombinant plasmid into a yeast peptone glucose (YPG) culture medium, inducing and culturing, collecting thalli, and washing to obtain the saccharomyces cerevisiae display linoleic acid isomerase; adding the obtained saccharomyces cerevisiae display linoleic acid isomerase into a buffer solution containing linoleic acid, and performing oscillating reaction. The linoleic acid isomerase is displayed and expressed on the outer surface of the cell of the saccharomyces cerevisiae, and is directly contacted with the linoleic acid substrate, so that the conversion efficiency of the conjugated linoleic acid is improved and the production cost is reduced.
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