http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102181519-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44 |
filingDate | 2011-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9af962ece87b85d449cbacb8c452efe0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a172e340b9df8858f8df1894c4b2463 |
publicationDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102181519-B |
titleOfInvention | Method for detecting gene polymorphism SNP (single nucleotide polymorphism) site by utilizing cyclization of RNase H (ribonuclease H) |
abstract | The invention relates to a method for detecting a gene polymorphism SNP (single nucleotide polymorphism) site by utilizing cyclization of RNase H (ribonuclease H), which belongs to the technical field of molecular biology. Transpeptidase Sortase A is utilized for cyclization of the linear RNase H, and a probe in the loop-stem structure, a DNA (deoxyribonucleic acid) fragment to be detected, a forward primer and a reverse primer of DNA specificity to be detected, a Vent DNA polymerase and cyclized APERNase H II are further utilized for measuring the specific site SNP of the DNA fragment to be detected, thereby improving the heat stability of the RNase H. |
priorityDate | 2011-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 143.