http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102181471-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78160fe5924d399deb065f8cbc5ad347 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-89 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 |
| filingDate | 2011-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b55ed1938e4b1e1dc8b7fb36219bf96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0dcc90ee5fe99381f74a42ed1bb3c2d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f79b3b1e3bb9595b81c2500a1cac064 |
| publicationDate | 2014-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102181471-B |
| titleOfInvention | Method for constructing secretory expression carrier and secretory expression system of Chlammydomonas reinhardtii |
| abstract | The invention discloses a method for constructing a secretory expression carrier and secretory expression system of Chlammydomonas reinhardtii. In the invention, the method comprises the steps of predicating a signal peptide gene sequence by using an amino acid sequence from a secretory protein of the Chlammydomonas reinhardtii through signal peptide predicating software; artificially synthesizing a signal peptide gene obtained by predicating; and inserting the signal peptide gene between a promoter and a cloning site of the Chlammydomonas reinhardtii expression carrier to construct the secretory expression carrier of the Chlammydomonas reinhardtii; meanwhile, detecting the expression efficiency of the constructed secretory expression carrier of the Chlammydomonas reinhardtii by using an exogenous gene; converting the secretory expression carrier of the Chlammydomonas reinhardtii, containing the exogenous gene, to the Chlammydomonas reinhardtii by using a Chlammydomonas reinhardtii genetic conversion method; and screening the Chlammydomonas reinhardtii to obtain a transgene engineering strain capable of expressing the exogenous gene in a secretory way. By using the invention, the basis is laid for using the Chlammydomonas reinhardtii as the secretory expression exogenous gene of a bioreactor. |
| priorityDate | 2011-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 96.