http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102181397-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7bae7cf399c53b51787e575edaf8b820 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 |
| filingDate | 2011-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1069afa3f3fa8d2e58fec9085605c826 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e09c4a5e026e34448d9b2eec19daeb7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bc75646d1078f742a41ce4d9b8c9ad1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e67880d0a359cca2ce985cbd08b68b1a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_238a55836dccf23a1b24e449e732d0da |
| publicationDate | 2014-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102181397-B |
| titleOfInvention | Laryngeal squamous cell carcinoma cartilage invasion model |
| abstract | The invention discloses a laryngeal squamous cell carcinoma cartilage invasion model. The laryngeal squamous cell carcinoma cartilage invasion model comprises a laryngeal squamous cell carcinoma invasion in-vitro experimental model, a laryngeal squamous cell carcinoma cartilage invasion semi-vitro experimental model, and a laryngeal squamous cell carcinoma cartilage invasion in-vivo experimental model. A method comprises the following steps of: closing the cup bottom of a Transwell chamber by using a polycarbonate microporous filter membrane; artificially reconstructing a basement membrane Matrigel and coating on the filter membrane; putting the Transwell chamber into a culture hole after the Matrigel is in a gel state, irradiating for 2h by using ultraviolet rays, and sterilizing for later use; adding an RPMI1640 culture medium containing 10 percent calf serum into the lower chamber, uniformly adding Hep-2 cells of laryngeal squamous cell carcinoma into the upper chamber, culturing, softly wiping away the Matrigel gel and cells which are not infiltrated at the cup bottom, immobilizing by using ethanol, and dyeing by using crystal violet; and counting the number of member-penetrating cells and taking an average value, wherein the relative number of the member-penetrating cells represents the invasiveness. In the invention, the laryngeal squamous cell carcinoma cartilage invasion model can be effectively established; and the discussion of an invasion mechanism of laryngeal squamous cell carcinoma on cartilages plays an important role in preventing the metastasis and relapse of laryngeal squamous cell carcinoma and selecting effective personalized therapeutic schedules. |
| priorityDate | 2011-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.