http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102174564-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8c111fdc57d222cc45810518afeb065c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60966d0f2d12b57d0b926eed4131e64b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac7d044c578c6ccc945fb3ac6f6a3a41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db1e220dea4e0ece74e3bcb15acf296f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3976b2df388ec0f01e474bf408386e57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef6647fe09ec5c44dcc251a19ad80f28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10e2f4a00fa6ed25d1ad0daeeccb128c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98bda1c2a5c2bf692cc4d2de7ed19d1a |
| publicationDate | 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102174564-B |
| titleOfInvention | Method for obtaining genetically modified sea island cotton |
| abstract | The invention discloses a method for obtaining genetically modified sea island cotton. The method comprises the following steps: preparing corresponding agrobacterium fluid, centrifuging and recovering the agrobacterium fluid, and adding a 1/2 MS (Murashige and Skoog) culture medium, 20% of cane sugar, 0.01% (v/v) of Selwet and 0.01% (v/v) of DMSO (dimethylsulfoxide) to ensure the OD (optical identity) value to reach around 0.6; placing the mixture in a small sprinkling can and spraying a sea island cotton flower which unfolds in the morning; after spraying, covering the flower with a paper tube and marking the flower; after seeds become ripe, planting a transformation T0 substitute to carry out gene marker detection and PCR (polymerase chian reaction) detection. The method has the advantages of simplicity in operation, freedom from restriction of genotype, high transformation efficiency, reduced workload and capabilities of promoting researches on gene transformation of the sea island cotton and accelerating molecular breeding process. At present, no reports on transformation of the sea island cotton agrobacterium by the infection method could be found. |
| priorityDate | 2011-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.