http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102154503-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3dcb0cef5f880e688b464211f4d2ddef |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8037b4d6a755ad5e82c5005253fef4a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7da86b734867e3c29c999b6a29c4ff35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd9ff491a708a032cc7a0531a248c2ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_735fb3882b34791949ef309eb8e9daec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4278581db1e5839309c4927ddf150246 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ac1faf3911c4429e7d29c9311c341fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bf6f833145b35ae746af0b87081643a |
| publicationDate | 2013-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102154503-B |
| titleOfInvention | Method for detecting DNA (deoxyribonucleic acid) content of CHO (cholesterol) cells by probe |
| abstract | The invention discloses a method for detecting DNA (deoxyribonucleic acid) content of CHO (cholesterol) cells by a probe, which comprises the following steps of: adopting a nucleotide sequence in a gene bank with accession number of EF 540878 so as to design a specific primer and a Taqman probe, carrying out the real-time fluorescence quantitative PCR (polymerase chain reaction) after extracting the DNA (deoxyribonucleic acid) from a sample under test, and obtaining the DNA (deoxyribonucleic acid) content of the CHO (cholesterol) cells in the sample under test by calculation according to an obtained standard curve. The invention further discloses a kit based on the method. The invention can accurately and quantificationally detect the residual DNA (deoxyribonucleic acid) content of CHO (cholesterol) cells in products derived from the CHO (cholesterol) cells such as therapeutic protein drugs, recombinant vaccines, monoclonal antibodies and the like. The method also provides an internal reference for analyzing copy numbers of specific genes in the CHO (cholesterol) cells. |
| priorityDate | 2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.