http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102154442-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate | 2010-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102154442-B |
| titleOfInvention | Method for detecting 1,5-anhydro sorbitol and related diagnostic kit |
| abstract | The invention relates to a method for detecting 1,5-anhydro sorbitol and a related diagnostic kit. In the method, a sample and adenosine diphosphate are reacted in the presence of adenosine diphosphate dependent hexokinase serving as a catalyst to form anhydroglucose-6-phosphoric acid and adenylic acid, the anhydroglucose-6-phosphoric acid and thio-oxidative NAD(nicotinamide adenine dinucleotide) are reacted in the presence of 1,5-AG(anhydro glucitol)-6-phosphate dehydrogenase(PDH) serving as a catalyst to form thio-reduced coenzymes and compounds of C6H11O8P, and simultaneously the generated C6H11O8P is reacted in the presence of AG-6-PDH and reduced NAD to form AG-6-P and oxidative coenzymes; and because of the cyclic amplification, the sensitivity of 6-anhydro sorbitol is improved. The invention also provides a detection kit for 1,5-anhydro sorbitol based on the method. The kit is convenient and easy to use, can quickly finish detection on a common ultraviolet/visible light analyzer or semi-/full-automatic biochemical analyzer, does not need special or additional instruments and is low in cost. |
| priorityDate | 2010-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 423.